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Q-NAD BLOOD NADP+ AND NADPH ASSAY KIT

Q40 NADP+ & NADPH Blood Kit (RUO)

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RUO Quantitative Assay Kit for Whole Blood

  • Highly accurate and sensitive
  • Easy to handle
    • One step metabolite extraction is followed by separate measurement of NADP+ and NADPH in the extract. 
    • Detection time is less than 10 minutes.
  • Higher throughput
    • Can be easily adopted into higher-throughput 96-well plate assay to analyze hundreds of samples per day.
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Notice: By placing an order for assay kits, you acknowledge that the kit requires standard laboratory equipment (See Kit Requirements specified below). Orders are non-returnable if the necessary equipment is not available in your laboratory (See Terms and Conditions for more information).

Key FeaturesInstructions for UseKit RequirementsPrinciplesProvided ReagentsPrecautions and Warnings

Key Features

Catalog number / nameRUO_004 / Q-NAD Blood NADP+ and NADPH assay kit
Detection methodColorimetric (OD570-573nm)
Assay typeQuantitative
Samples typeWhole blood
SpeciesAll
Sample volume120 μL (with K2 EDTA or heparin)
Metabolites measuredNADP+, NADPH
Detection Limit400 nM for NADP+ and 300 nM NADPH in the whole blood
Assay Length< 1h
Size192 reactions
Number of samples40 (measured in duplicates)
StorageStore reagents at -80°C
Intended useFor Research Use Only
Shelf Life12 months from the production date

Instructions for Use

EN – Vs. 1, Last modified August 2025


Troubleshooting

Certificate of Analysis

Kit Requirements

  • Multi-channel pipets
  • Two clear-bottom 96-well plates suitable for colorimetric assays
  • Heat block with adjustable temperature (up to 80°C)
  • Spectrophotometric plate reader

Principles

The principle of the assay is a cyclic enzymatic reaction with a colorimetric end-point detection. First, NADP+ and NADPH metabolites are extracted together from a blood sample in a single step. Then the extract is divided into two parts revealing both NADP+ and NADPH concentrations by removing one metabolite and stabilizing the other.

Provided Reagents

  • Extraction BUFFER A
  • NADP+ stabilizing reagent
  • NADPH stabilizing reagent
  • NADP+ standard stock
  • NADPH standard stock
  • Assay BUFFER C
  • Assay color reagent
  • Enzyme
  • Stop solution
  • Positive control

Precautions and Warnings

  • For research use only by trained personnel.
  • The Stop Solution may cause skin, eye, and respiratory irritation. Avoid breathing in fumes.
  • Assay color reagent may cause skin irritation. Handle with care, use gloves.
  • BUFFER A can cause eye irritation. Handle with care, use googles.

Read more in Q-NADMED Safety Data Sheet (SDS)

EN – Vs. 7, Last modified May 2025

DE – Vs. 7, Last modified May 2025

ES – Vs. 7, Last modified May 2025 

More details how to prepare samples in the sample preparation document (vs. 2, last modified September 2023).