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Q-NAD TISSUE/CELLS NAD+ AND NADH ASSAY KIT

Q40 NAD+ & NADH Tissue / Cell Kit (RUO)

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RUO Quantitative Assay Kit for Tissues and Cells

  • Highly accurate and sensitive
  • Easy to handle
    • One step metabolite extraction is followed by separate measurement of NAD+ and NADH in the extract. 
    • Detection time is less than 10 minutes.
  • Higher throughput
    • Can be easily adopted into higher-throughput 96-well plate assay to analyze hundreds of samples per day.
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Notice: By placing an order for assay kits, you acknowledge that the kit requires standard laboratory equipment (See Kit Requirements specified below). Orders are non-returnable if the necessary equipment is not available in your laboratory (See Terms and Conditions for more information).

Key FeaturesInstructions for UseKit RequirementsPrinciplesProvided Reagents Precautions and Warnings

Key Features

Catalog number / nameRUO_003 / Q-NAD Tissue/Cell  NAD+ and NADH assay kit
Detection methodColorimetric (OD570-573nm)
Assay typeQuantitative
Samples type Tissues / Cells
SpeciesAll
Guideline for Sample volume 20 mg of tissue per 1 mL of extraction buffer 0.5 M cells per 100 μL of extraction buffer
Metabolites measuredNAD+, NADH
Detection Limit 25 nM for NAD+ and 35 nM NADH in the cell/tissue extract
Assay Length< 1h
Size192 reactions
Number of samples40 (measured in duplicates)
StorageStore reagents at -80°C
Intended useFor Research Use Only
Shelf Life12 months from the production date

Instructions for Use

EN – Vs. 4, Last modified September 2024

Troubleshooting

Certificate of Analysis

Kit Requirements

  • Multi-channel pipets
  • Two clear-bottom 96-well plates suitable for colorimetric assays
  • Heat block with adjustable temperature (up to 80°C)
  • Spectrophotometric plate reader

Principles

The principle of the assay is a cyclic enzymatic reaction with a colorimetric end-point detection. First, NAD+ and NADH metabolites are extracted together from a tissue or cell culture sample in a single step. Then the extract is divided into two parts revealing both NAD+ and NADH concentrations by removing one metabolite and stabilizing the other.

Provided Reagents 

  • Extraction BUFFER A
  • NAD+ stabilizing reagent
  • NADH stabilizing reagent
  • NAD+ standard stock
  • NADH standard stock
  • BUFFER C
  • BUFFER D
  • Assay color reagent
  • Enzyme
  • Stop solution
  • Positive control

Precautions and Warnings

  • For research use only by trained personnel.
  • The Stop Solution may cause skin, eye, and respiratory irritation. Avoid breathing in fumes.
  • Assay color reagent may cause skin irritation. Handle with care, use gloves.
  • BUFFER A can cause eye irritation. Handle with care, use goggles.

Read more in Safety Data Sheet (SDS) - EN – Vs. 4, Last modified January 2024

More details how to prepare samples in sample preparation document. Protocol for cell collection with NADMED lysis buffer you can find here (vs. 2, Last update April 2024).