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Q-NAD BLOOD NAD+ AND NADH ASSAY KIT (US) - SMALL

Q16 NAD+ & NADH Small Blood Kit (RUO), Americas

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RUO Quantitative Assay Kit for Whole Blood

Highly accurate and sensitive
  • Quantitation limit is 330 nM for NAD+ and 119 nM NADH in whole blood measured in two 96-well plate assays.
Easy to handle
  • One step metabolite extraction is followed by separate measurement of NAD+ and NADH in the extract. 
  • Detection time is less than 10 minutes.
Higher throughput
  • Can be easily adopted into higher-throughput 96-well plate assay to analyze hundreds of samples per day.
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Notice: By placing an order for assay kits, you acknowledge that the kit requires standard laboratory equipment (See Kit Requirements specified below). Orders are non-returnable if the necessary equipment is not available in your laboratory (See Terms and Conditions for more information).

Key FeaturesInstructions for UseKit RequirementsPrinciplesProvided ReagentsPrecautions and Warnings

Key Features

Catalog number / nameRUO_001_S_US / Q-NAD Blood NAD+ and NADH assay kit (US) - Small
Detection methodColorimetric (OD570-573nm)
Assay typeQuantitative
Samples typeWhole blood
SpeciesAll
Sample volume120 μL (with K2 EDTA or heparin)
Metabolites measuredNAD+, NADH
Detection Limit330 nM for NAD+ and 119 nM NADH in the whole blood
Assay Length< 1h
Size100 reactions
Number of samples16 (measured in duplicates)
StorageStore reagents at -80°C
Intended useFor Research Use Only
Shelf Life12 months from the production date

Instructions for Use

EN – will be available soon


Troubleshooting

Certificate of Analysis

Kit Requirements

  • Multi-channel pipets
  • Two clear-bottom 96-well plates suitable for colorimetric assays
  • Heat block with adjustable temperature (up to 80°C)
  • Spectrophotometric plate reader

Principles

The principle of the assay is a cyclic enzymatic reaction with a colorimetric end-point detection. First, NAD+ and NADH metabolites are extracted together from a blood sample in a single step. Then the extract is divided into two parts revealing both NAD+ and NADH concentrations by removing one metabolite and stabilizing the other.

Provided Reagents

  • Extraction BUFFER A
  • NAD+ stabilizing reagent
  • NADH stabilizing reagent
  • NAD+ standard stock
  • NADH standard stock
  • Assay BUFFER C
  • Assay color reagent
  • Enzyme
  • Stop solution
  • Positive control

Precautions and Warnings

  • For research use only by trained personnel.
  • The Stop Solution may cause skin, eye, and respiratory irritation. Avoid breathing in fumes.
  • Assay color reagent may cause skin irritation. Handle with care, use gloves.
  • BUFFER A can cause eye irritation. Handle with care, use googles.

Read more in Q-NADMED Safety Data Sheet (SDS)

EN – will be available soon 

More details how to prepare samples in the sample preparation document (vs. 2, last modified September 2023).