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Q-NAD Blood NAD+ and NADH Assay kit

Q40 NAD+ & NADH Blood Kit (RUO)

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Catalog name: Q-NAD Blood NAD+ and NADH Assay kit 

RUO Quantitative 40-sample Assay Kit for Whole Blood

Highly accurate and sensitive
  • Quantitation limit is 330 nM for NAD+ and 119 nM NADH in whole blood measured in two 96-well plate assays.
Easy to handle
  • One step metabolite extraction is followed by separate measurement of NAD+ and NADH in the extract. 
  • Detection time is less than 10 minutes.
Higher throughput
  • Can be easily adopted into higher-throughput 96-well plate assay to analyze hundreds of samples per day.
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Notice: By placing an order for assay kits, you acknowledge that the kit requires standard laboratory equipment (See Kit Requirements specified below). Orders are non-returnable if the necessary equipment is not available in your laboratory (See Terms and Conditions for more information).

Key FeaturesInstructions for UseKit RequirementsPrinciplesProvided ReagentsStoragePrecautions and Warnings

Key Features

Catalog number / nameRUO_001 / Q-NAD Blood NAD+ and NADH Assay kit
Detection methodColorimetric (OD570-573nm)
Assay typeQuantitative
Samples typeWhole blood
SpeciesAll
Sample volume120 μL (with K2 EDTA or heparin)
Metabolites measuredNAD+, NADH
Detection Limit330 nM for NAD+ and 119 nM NADH in the whole blood
Assay Length< 1h
Size192 reactions
Number of samples40 (measured in duplicates)
StorageStore reagents at -80°C
Intended useFor Research Use Only
Shelf Life12 months from the production date

Instructions for Use

Find the IFU under Q-NAD Blood NAD+ and NADH Assay kit / Q40 NAD+ & NADH Blood Kit (RUO) here.


If you experience any issues, please refer to the troubleshooting guide.

Certificate of Analysis is available upon request.
Please contact your NADMED representative or email us at info@nadmed.com. We will be happy to provide it.

Kit Requirements

  • Multi-channel pipets
  • Two clear-bottom 96-well plates suitable for colorimetric assays
  • Heat block with adjustable temperature (up to 80°C)
  • Spectrophotometric plate reader

Principles

The principle of the assay is a cyclic enzymatic reaction with a colorimetric end-point detection. First, NAD+ and NADH metabolites are extracted together from a blood sample in a single step. Then the extract is divided into two parts revealing both NAD+ and NADH concentrations by removing one metabolite and stabilizing the other.

Provided Reagents

  • EXTRACTION BUFFER A
  • NAD+ STABILIZER
  • NADH STABILIZER
  • NAD+ STANDARD STOCK
  • NADH STANDARD STOCK
  • ASSAY BUFFER C
  • ASSAY COLOR REAGENT
  • NAD ENZYME
  • STOP SOLUTION
  • POSITIVE CONTROL
  • DEIONIZED WATER

Storage

If you are storing the kits for more than one month, place them in a freezer set to -80°C. If you plan to run the assay within four weeks, the kits may be stored at stable -20°C.

Precautions and Warnings

  • For research use only by trained personnel.
  • The STOP SOLUTION may cause skin, eye, and respiratory irritation. Avoid breathing in fumes.
  • ASSAY COLOR REAGENT may cause skin irritation. Handle with care, use gloves.
  • EXTRACTION BUFFER A can cause eye irritation. Handle with care, use goggles.

For more information, please refer to the Safety Data Sheet (SDS) for the blood kit and for more details on sample preparation, see the Blood preparation instructions, both available here.