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Q-NADMED BLOOD NAD+ AND NADH ASSAY KIT - SMALL

Q16 NAD+ & NADH Small Blood Kit (CE)

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CE-IVD Quantitative Assay Kit for Whole Blood

Highly accurate and sensitive
  • Quantitation limit is 330 nM for NAD+ and 119 nM NADH in whole blood measured in two 96-well plate assays.
Easy to handle
  • One step metabolite extraction is followed by separate measurement of NAD+ and NADH in the extract.
  • Detection time is less than 10 minutes.
Higher throughput
  • Can be easily adopted into higher-throughput 96-well plate assay to analyze hundreds of samples per day.
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Notice: By placing an order for assay kits, you acknowledge that the kit requires standard laboratory equipment (See Kit Requirements specified below). Orders are non-returnable if the necessary equipment is not available in your laboratory (See Terms and Conditions for more information).

Key FeaturesInstructions for UseKit RequirementsIntended UsePrinciplesProvided ReagentsPrecautions and Warnings

Key Features

Catalog number / nameIVD_001_S / Q-NADMED Blood NAD+ and NADH assay kit - Small
Detection methodColorimetric (OD570-573nm)
Assay typeQuantitative
Samples typeWhole blood
SpeciesAll
Sample volume120 μL (with K2 EDTA or heparin)
Metabolites measuredNAD+, NADH
Detection Limit330 nM for NAD+ and 119 nM NADH in the whole blood
Assay Length< 1h
Size100 reactions
Number of samples16 (measured in duplicates)
StorageStore reagents at -80°C
Intended useCE-IVD
Shelf Life12 months from the production date

Instructions for Use

EN – will be available soon


Troubleshooting

Certificate of Analysis

Kit Requirements

  • Multi-channel pipets
  • Two clear-bottom 96-well plates suitable for colorimetric assays
  • Heat block with adjustable temperature (up to 80°C)
  • Spectrophotometric plate reader

Intended Use

Q-NADMED Blood, an in-vitro diagnostic medical device, is an analytical assay kit for measurement of concentrations of NAD+ and NADH metabolites in human whole blood. The assay is quantitative. Intended users of the Q-NADMED assay kit are trained laboratory personnel.

Principles

The principle of the assay is a cyclic enzymatic reaction with a colorimetric end-point detection. First, NAD+ and NADH metabolites are extracted together from a blood sample in a single step. Then the extract is divided into two parts revealing both NAD+ and NADH concentrations by removing one metabolite and stabilizing the other.

Provided Reagents

  • Extraction BUFFER A
  • NAD+ stabilizing reagent
  • NADH stabilizing reagent
  • NAD+ standard stock
  • NADH standard stock
  • Assay BUFFER C
  • Assay color reagent
  • Enzyme
  • Stop solution
  • Positive control

Precautions and Warnings

  • For research use only by trained personnel.
  • The Stop Solution may cause skin, eye, and respiratory irritation. Avoid breathing in fumes.
  • Assay color reagent may cause skin irritation. Handle with care, use gloves.
  • BUFFER A can cause eye irritation. Handle with care, use googles.

Read more in Q-NADMED Safety Data Sheet (SDS)

EN – will be available soon

More details how to prepare samples in the sample preparation document (vs. 2, last modified September 2023).