Q16 NAD+ & NADH Small Blood Kit (CE)

Catalogue name: Q-NADMED Blood NAD+ and NADH Assay kit - SMALL

CE-IVD Quantitative 16-sample Assay Kit for Whole Blood

Highly accurate and sensitive

Quantitation limit is 330 nM for NAD+ and 119 nM NADH in whole blood measured in two 96-well plate assays.

Easy to handle

One step metabolite extraction is followed by separate measurement of NAD+ and NADH in the extract.
Detection time is less than 10 minutes.

Higher throughput

Can be easily adopted into higher-throughput 96-well plate assay to analyze hundreds of samples per day.

Notice: By placing an order for assay kits, you acknowledge that the kit requires standard laboratory equipment (See Kit Requirements specified below). Orders are non-returnable if the necessary equipment is not available in your laboratory (See Terms and Conditions for more information).

Key Features

Catalog number / name	IVD_001_S / Q-NADMED Blood NAD+ and NADH Assay kit - SMALL
Detection method	Colorimetric (OD570-573nm)
Assay type	Quantitative
Samples type	Whole blood
Species	All
Sample volume	120 μL (with K2 EDTA or heparin)
Metabolites measured	NAD+, NADH
Detection Limit	330 nM for NAD+ and 119 nM NADH in the whole blood
Assay Length	< 1h
Size	100 reactions
Number of samples	16 (measured in duplicates)
Storage	Store reagents at -80°C
Intended use	CE-IVD
Shelf Life	12 months from the production date

Instructions for Use

Find the IFU under Q-NADMED Blood NAD+ and NADH Assay kit - SMALL / Q16 NAD+ & NADH Small Blood Kit (CE) here.

If you experience any issues, please refer to the troubleshooting guide.

Certificate of Analysis is available upon request.
Please contact your NADMED representative or email us at info@nadmed.com. We will be happy to provide it.

Kit Requirements

Multi-channel pipets
Two clear-bottom 96-well plates suitable for colorimetric assays
Heat block with adjustable temperature (up to 80°C)
Spectrophotometric plate reader

Intended Use

Q-NADMED Blood NAD+ and NADH Assay kit – SMALL, an in-vitro diagnostic medical device, is an analytical assay kit for measurement of concentrations of NAD+ and NADH metabolites in human whole blood. The assay is quantitative. Intended users of the assay kit are trained laboratory personnel.

Principles

The principle of the assay is a cyclic enzymatic reaction with a colorimetric end-point detection. First, NAD+ and NADH metabolites are extracted together from a blood sample in a single step. Then the extract is divided into two parts revealing both NAD+ and NADH concentrations by removing one metabolite and stabilizing the other.

Provided Reagents

EXTRACTION BUFFER A – SMALL
NAD+ STABILIZER
NADH STABILIZER
NAD+ STANDARD STOCK
NADH STANDARD STOCK
ASSAY BUFFER C – SMALL
ASSAY COLOR REAGENT – SMALL
NAD ENZYME
STOP SOLUTION
POSITIVE CONTROL
DEIONIZED WATER

Storage

If you are storing the kits for more than one month, place them in a freezer set to -80°C. If you plan to run the assay within four weeks, the kits may be stored at stable -20°C.

Precautions and Warnings

For in vitro diagnostic use only by trained personnel.
The STOP SOLUTION may cause skin, eye, and respiratory irritation. Avoid breathing in fumes.
ASSAY COLOR REAGENT – SMALL may cause skin irritation. Handle with care, use gloves.
EXTRACTION BUFFER A – SMALL can cause eye irritation. Handle with care, use goggles.

For more information, please refer to the Safety Data Sheet (SDS) for the blood kit and for more details on sample preparation, see the Blood preparation instructions, both available here.

Terms and Conditions